[发明专利]草鱼肠道细胞胞质苹果酸脱氢酶基因序列

摘要

本发明公开了一种草鱼肠道细胞胞质苹果酸脱氢酶的基因，以本实验室保存草鱼肠道cDNA文库中胞质苹果酸脱氢酶EST序列为模版设计引物，结合RACE技术，用PCR法扩增克隆得到草鱼肠道细胞胞质苹果酸脱氢酶基因的全表达序列。对于研究草鱼肠道细胞胞质苹果酸脱氢酶基因结构组成，探讨其在草鱼肠道代谢调控中的作用和功能区域位置，以及与其他物种的区别都提供了重要的理论依据。本发明对探索建立草鱼基因组计划与功能饲料产业化之间纽带的方法具有开创性作用。

主权项

1、一种草鱼草鱼肠道细胞胞质苹果酸脱氢酶基因，其特征在于具有以下RNA核苷酸及相应的氨基酸序列：gctgagtggc agagcagcac atctttggcg cacatctcaa cctgcacaat cagacttgaa 60ac atg gcc gaa ccg atc cgt gtc ctg gtg act 92Met Ala Glu Pro Ile Arg Val Leu Val Thr 101 5 10ggc gca gcc gga cag atc gcc tat tct ctg 122Gly Ala Ala Gly Gln Ile Ala Tyr Ser Leu 2011 15 20ctc tac agc att gct aaa gga gat gtg ttc 152Leu Tyr Ser Ile Ala Lys Gly Asp Val Phe 3021 25 30ggc aag gac cag cca atc atc ttg gtg ctt 182Gly Lys Asp Gln Pro Ile Ile Leu Val Leu 4031 35 40ctg gac atc act ccc atg ctg cct gtg ctg 212Leu Asp Ile Thr Pro Met Leu Pro Val Leu 5041 45 50gat ggg gtc gtc atg gaa ctg cag gat tgt 242Asp Gly Val Val Met Glu Leu Gln Asp Cys 6051 55 60gct ctt cct ctt ctg agg gat gtg att cct 272Ala Leu Pro Leu Leu Arg Asp Val Ile Pro 7061 65 70act gat aag gtt gag gtg ggc ttc aag gac 302Thr Ala Lys Val Glu Val Gly Phe Lys Asp 8071 75 80ctt gat gct gcc atc ttg gtg ggc tct atg 332Leu Asp Ala Ala Ile Leu Val Gly Ser Met 9081 85 90cca agg aaa gag ggc atg gag aga aag gac 362Pro Arg Lys Glu Gly Met Glu Arg Lys Asp 10091 95 100ctt ctg aag gcc aac gtg gcc att ttt aaa 392Leu Leu Lys Ala Asn Val Ala Ile Phe Lys 110101 105 110acc caa ggt gaa gca ctg gag aag tac gcc 422Thr Gln Gly Glu Ala Leu Glu Lys Tyr Ala 120111 115 120cag aag act gtc aag gtg cta gtt gtc ggg 452Gln Lys Thr Val Lys Val Leu Val Val Gly 130121 125 130aat cca gcc aat acc aac tgt ttg atc gcc 482Asn Pro Ala Asn Thr Asn Cys Leu Ile Ala 140131 135 140tcc aaa tct gct ccg tcc att cct aag gag 512Ser Lys Ser Ala Pro Ser Ile Pro Lys Glu 150141 145 150aac ttc tcc tgc ctg acc cgt ctg gac cat 542Asn Phe Ser Cys Leu Thr Arg Leu Asp His 160151 155 160aac agg gcc cgc tct cag gtg gcg atg cgt 572Asn Arg Ala Arg Ser Gln Val Ala Met Arg 170161 165 170gtt ggt gtg tcc tct gac aat gtg aag aat 602Val Gly Val Ser Ser Asp Asn Val Lys Asn 180171 175 180gtg att atc tgg gga aat cac tcc tca act 632Val Ile Ile Trp Gly Asn His Ser Ser Thr 190181 185 190cag tac cca gat gtg cac cac gct atc gtg 662Gln Tyr Pro Asp Val His His Ala Ile Val 200191 195 200aac cac cat ggg aag gag ttg gca gcc ttt 692Asn His His Gly Lys Glu Leu Ala Ala Phe 210201 205 210gac gcc gtg aat gat gaa agc tgg ctg aag 722Asp Ala Val Asn Asp Glu Ser Trp Leu Lys 220211 215 220ggc gac ttc atc tcc acg gtg cag cag aga 752Gly Asp Phe Ile Ser Thr Val Gln Gln Arg 230221 225 230ggt gca gct gtc atc aag gcc agg aag ctc 782Gly Ala Ala Val Ile Lys Ala Arg Lys Leu 240231 235 240tcc agc gca atg tct gct gcc aaa gcc atc 812Ser Ser Ala Met Ser Ala Ala Lys Ala Ile 250241 245 250tgt gac cac atg agg gac atc tgg ttc ggc 842Cys Asp His Met Arg Asp Ile Trp Phe Gly 260251 255 260act cct gat ggt gag tgg gtg tct atg ggc 872Thr Pro Asp Gly Glu Trp Val Ser Met Gly 270261 265 270gtc tac tcc tct ggt aat tcc tat gga gtt 902Val Tyr Ser Ser Gly Asn Ser Tyr Gly Val 280271 275 280cct gat gat ctc atg tac tcc ttc cct gtt 932Pro Asp Asp Leu Met Tyr Ser Phe Pro Val 290281 285 290aag att aag aac aag acc tgg aag gtg gtt 962Lys Ile Lys Asn Lys Thr Trp Lys Val Val 300291 295 300gac gga ctc tcc atc aac gat ttc tcc cgc 992Asp Gly Leu Ser Ile Asn Asp Phe Ser Arg 310301 305 310gct aag atg gac gcc acc tcc gct gag ctg 1022Ala Lys Met Asp Ala Thr Ser Ala Glu Leu 320311 315 320gta gag gag aga gac acg gca gtc acc ttc 1052Val Glu Glu Arg Asp Thr Ala Val Thr Phe 330321 325 330ctt gga gcg 1061Leu Gly Ala 333331 333tgactcgact ccacgacaac accgcagcag ttagaccctt caaagggccc aaatccccga 1121ctgtagaatt cacagctgac cgtgtgtgtg tgtgtgtgtg tgtgtggata ttcatgtatc 1181ttaacgtatg cgtgtgtgcg tgtgtaactt ctcaagaaac gtttgcaatg taacttactc 1241tgcttggagt gtccggggca acacctgaag gagaaccttt ccaattataa ccgttacgca 1301catggttgtc ctgaccaaaa tgatgatcat ctgtcataca acaataaaag tacatgggaa 1361aaaaaaaaaa aaaaaaaaaa aaaaactcga 1391